Colchicine

SKU: orb1310726

Description

Colchicine is a potent natural alkaloid that inhibits microtubule polymerization (IC50 = 3 nM) and acts as a competitive glycine receptor antagonist. It is widely used in research for its anti-inflammatory and immunosuppressive properties, applied in studies of gout, rheumatoid arthritis, Alzheimer's disease models, and NLRP3 inflammasome-related pathologies in both cellular and animal studies.

Research Area

Cell Biology, Signal Transduction

Images & Validation

−

Key Properties

−

CAS Number	64-86-8
MW	399.44
Purity	99.96% (May vary between batches)
Formula	C₂₂H₂₅NO₆
SMILES	COC1=C(OC)C(OC)=C2C(CCC(NC(C)=O)C3=CC(=O)C(OC)=CC=C23)=C1
Target	Microtubule Associated,Apoptosis,Autophagy
Solubility	H2O:1.33 mg/mL (3.33 mM);DMSO:257.5 mg/mL (644.65 mM)

Bioactivity

−

Target IC50	A10 cells:0.03 μM (EC50)\|Microtubule:3 nM\|786-O cells:0.022 μM
In Vivo	METHODS: To investigate the antitumor activity, Colchicine (0.1 mg/kg) was orally administered to BALB/c-nu mice bearing the human pharyngeal cancer tumor FaDu every two days for fourteen days. RESULTS: Colchicine was effective in inhibiting tumor growth in a hypopharyngeal cancer model nude mouse without serious complications. METHODS: To investigate the effect of anti-Fas antibody-induced lethality, Colchicine (2 mg/kg) was injected intraperitoneally into C57BL/6 mice, followed by Jo2 antibody (10 μg) 24 h later. RESULTS: All mice treated with Colchicine survived the lethal attack.Colchicine reduced the susceptibility of mice to the lethal effect of Jo2 against Fas antibody. Read more...
In Vitro	METHODS: Human pharyngeal carcinoma cells FaDu and SNU1041 were treated with Colchicine (0.0-1 μM) for 24-72 h. Cell viability was measured by XTT assay. RESULTS: Colchicine treatment was cytotoxic to both FaDu and SNU1041 cell lines in a dose- and time-dependent manner. METHODS: Chorionic villous cells AFCs and amniotic fluid cells CVCs were treated with Colchicine (0.15 μg/mL) for 3-24 h. Apoptosis was detected by Flow Cytometry. RESULTS: Colchicine induced a significant increase in the proportion of annexin V and PI double positive cells. Read more...
Animal Research	a C57BL/6 background are used. To examine the effects of Colchicine on NSAID-induced small intestinal injury, vehicle or Colchicine (1 or 3 mg/kg) is administered orally 30 min prior to indomethacin administration. Mice received intraperitoneal injections of sterilized phosphate buffered saline or mouse recombinant IL-1β (0.1 μg/kg) 3 h after indomethacin treatment. Vehicle or Colchicine (1 or 3 mg/kg) is also administered to NLRP3 / mice before indomethacin administration. The lesion index is evaluated 24 h after indomethacin administration and examined mRNA and protein expression of inflammasome components 6 h after indomethacin administration. Read more...

Storage & Handling

−

Storage	keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

microtubule, MicrotubuleAssociated, Microtubule/Tubulin, Microtubule Associated, inhibit, Inhibitor, Apoptosis, Autophagy, Colchicine, Colchineos, Colchisol, Colcin

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID

2833 ↗

Key Properties

−

No computed properties available.

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Find More Protocols

Colchicine (orb1310726)

Based on 0 reviews

Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.

No reviews yet

Purity:	>98%
MW:	387.43
Formula:	C₂₁H₂₅NO₆

CAS Number:	3025180-14-4
MW:	444.458
Formula:	C₂₅H₂₁FN₄O₃

CAS Number:	3046118-64-0
MW:	423.34
Formula:	C₁₉H₂₁FN₂O₃Se

Purity:	99.91% (May vary between batches)
CAS Number:	1477482-50-0
MW:	282.34
Formula:	C₁₆H₁₈N₄O

Purity:	99.38% (May vary between batches)
CAS Number:	477-27-0
MW:	385.41
Formula:	C₂₁H₂₃NO₆

Colchicine

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

Similar Products

MY-875 [orb2279981]

Microtubulin-IN-1 [orb3028635]

Antitumor agent-200 [orb3028675]

Suprafenacine [orb1304885]

colchiceine [orb1984312]

Quality Guarantee

Compound Identifiers

Key Properties

Documents Download

Request a Document

Protocol Information

Colchicine (orb1310726)

Step 1: Enter information below

Step 2: Enter the in vivo formulation

Available Sizes

Email

UK Office

US Office

CN Office

Distributors