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Collagen IV Antibody / COL4A1/2
SKU: orb1151657
Description
Research Area
Cancer, Epigenetics
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution Range | Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | M3F7 |
| Immunogen | An amino acid sequence from the alpha1 (IV)-alpha2(IV) triple helix was used as the immunogen for the Collagen IV antibody. This antibody does not recognize denatured type IV collagen. |
| Purification | Protein A/G affinity |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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IHC staining of FFPE human kidney tissue with Collagen IV antibody (clone M3F7). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney tissue with Collagen IV antibody (clone M3F7). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney adenocarcinoma tissue with Collagen IV antibody (clone M3F7) at 2 ug/ml in PBS for 30 min RT. Strong staining of glomeruli is observed. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
Collagen IV Antibody / COL4A1/2 (orb1151657)
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