Collagen IV Antibody

SKU: orb1539266

This product can be custom made

Description

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

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Tested Applications	ICC, IHC, IHC-Fr, IHC-P, WB
Dilution Range	ICC, IHC, IHC-Fr (1:25 - 1:100), IHC-P (1:75 - 1:100), WB (1:100 - 1:1000)
Reactivity	Bovine, Human, Porcine, Rat
Application Notes	Further information: Methanol fixation recommended for immunocytochemistry. For immunohistochemistry on frozen tissues (fixed in methanol, aceton or 1% paraformaldehyde) a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 is recommended. For immunohistochemistry on paraffin tissue the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature.

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Full length Type IV collagen isolated from human placenta
Target	Collagen IV
Purification	Antiserum
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Antiserum, 0.09% sodium azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Quick Database Links

Gene Symbol

Collagen IV

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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ICC

Immunocytochemistry

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