Collagen IV Rabbit Polyclonal Antibody

SKU: orb1735106

Description

Images & Validation

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Item 1 of 4

Tested Applications	IF, IHC, WB
Dilution Range	Western Blot 1:1000 - 1:1500, Immunofluorescence 1:300 – 1:500, Immunohistochemistry (Paraffin) 1:100 – 1:200
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide
Target	Collagen IV /COL4A1
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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COL4A1 / Collagen IV antibody - at 1/1000 dilution. Lysates at 40 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked before being incubated with orb1735106 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human placenta labeling COL4A1 / Collagen IV with orb1735106 at 1/100.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling COL4A1 / Collagen IV with orb1735106 at 1/100.

Quick Database Links

Gene Symbol

Collagen IV /COL4A1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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