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Collagen Type IV, human Antibody

SKU: orb27211

Description

Mouse monoclonal antibody to Collagen IV

Research Area

Cell Biology

Images & Validation

Tested ApplicationsIHC-Fr, IHC-P, WB
Dilution Range(liquid conc.) 1:10 - 1:50
Application Notes
Positive Control: Human skin, placenta or lens capsule. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: 60 min at RT

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.COL 94
ImmunogenPurified human Collagen IV
PurificationAntibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Concentrationapp. 100 µg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol

Collagen Type IV, human Antibody (orb27211)

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1 ml
$ 600.00
DispatchUsually dispatched within 5-10 working days
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