Collagen VI Antibody

SKU: orb1538872

Description

Images & Validation

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Tested Applications	ELISA, IHC, IHC-P, IP, WB
Dilution Range	ELISA (1:5000 - 1:20000), IHC, IHC-P (2.5 µg/ml), IP (1:100), WB (1:500 - 1:5000)
Reactivity	Bovine, Human, Mammal
Application Notes	Further information: Percentage of reactivty with all isoforms of COL6 has not been determined. Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 2.5 ug/ml. Read more...

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Collagen Type VI from human and bovine placenta.
Target	Collagen VI
Purification	Affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Quick Database Links

Gene Symbol

Collagen VI

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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