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COX6A1 Antibody

SKU: orb1257822

Description

COX6A1 Antibody

Research Area

Neuroscience

Images & Validation

Tested ApplicationsIF, IHC, WB
ReactivityHuman, Mouse, Rat
Application Notes
WB: 1:200 - 1:2000IHC: 1:20 - 1:200IF: 1:50 - 1:200

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-109 of human COX6A1 (NP_004364.2).
TargetCOX6A1
Molecular WeightObserved: 12kDa
PurificationAffinity purification
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Cytochrome c oxidase subunit 6A1, mitochondrial, Cytochrome c oxidase polypeptide VIa-liver, Cytochrome c oxidase subunit VIA-liver, COX VIa-L, COX6A1, COX6AL

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COX6A1 Antibody

Immunofluorescence analysis of C6 cells using COX6A1 Polyclonal Antibody (orb1257822) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

COX6A1 Antibody

Western blot analysis of extracts of various cell lines, using COX6A1 antibody (orb1257822) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

COX6A1 Antibody

Immunohistochemistry of paraffin-embedded rat cerebellum using COX6A1 antibody (orb1257822) at dilution of 1:100 (40x lens).

COX6A1 Antibody

Immunohistochemistry of paraffin-embedded human liver cancer using COX6A1 antibody (orb1257822) at dilution of 1:100 (40x lens).

COX6A1 Antibody

Immunohistochemistry of paraffin-embedded mouse liver using COX6A1 antibody (orb1257822) at dilution of 1:100 (40x lens).

COX6A1 Antibody

Immunofluorescence analysis of NIH-3T3 cells using COX6A1 Polyclonal Antibody (orb1257822) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

COX6A1 Antibody

Immunofluorescence analysis of U-2 OS cells using COX6A1 Polyclonal Antibody (orb1257822) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol

COX6A1 Antibody (orb1257822)

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Available Sizes

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100 μl
$ 830.00
DispatchUsually dispatched within 5-10 working days
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