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Coxsackie Adenovirus Receptor Rabbit Polyclonal Antibody

SKU: orb4966
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Description

Coxsackie Adenovirus Receptor Rabbit Polyclonal Antibody

Research Area

Host-Virus Interaction, Signal Transduction, Virology

Images & Validation

Tested ApplicationsFC
Dilution rangeFlow-Cyt=1μg/Test
ReactivityHuman
Predicted ReactivityBovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Coxsackie Adenovirus Receptor (21-120/365aa)
TargetCXADR
Molecular Weight38 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

46 kD coxsackievirus and adenovirus receptor CAR protein; 46 kD coxsackievirus and adenovirus receptor protein; CAR; CAR4/6; Coxsackie virus and adenovirus receptor; Coxsackie virus B receptor; Coxsackievirus and adenovirus receptor; Coxsackievirus B adenovirus receptor; Coxsackievirus B-adenovirus receptor; CVB3 binding protein; CVB3-binding protein; Cxadr; CXAR_HUMAN; hCAR; HCVADR.

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Coxsackie Adenovirus Receptor Rabbit Polyclonal Antibody

Blank control (Blue): A549 (fixed with 2% paraformaldehyde for 10 min at 37°C). Primary Antibody: Rabbit Anti-Coxsackie Adenovirus Receptor antibody (orb4966, Green), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Coxsackie Adenovirus Receptor Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Coxsackie Adenovirus Receptor) Polyclonal Antibody, Unconjugated (orb4966) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

UniProt Details

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Protocol Information

FC
Flow Cytometry
View Protocol

Coxsackie Adenovirus Receptor Rabbit Polyclonal Antibody (orb4966)

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Available Sizes

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50 μl
$ 250.00
100 μl
$ 390.00
200 μl
$ 470.00
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