Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human CR2 (981-1092/1092aa)
Target	CR2
Molecular Weight	111 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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C3DR; CD 21; CD21; CD21 antigen; Complement C3d receptor; Complement component(3d/Epstein Barr virus) receptor 2; Complement component receptor 2; Complement receptor type 2; Cr 2; EBV receptor; Epstein Barr virus receptor; CR2_HUMAN.

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Blank control (blue): Molt-4 Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD21/AF647 Conjugated antibody (orb7143/AF647), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions.

Blank control (blue): U-87MG Cells (fixed with 2% paraformaldehyde (10 min). Primary Antibody: Rabbit Anti-CD21/AF647 Conjugated antibody (orb7143/AF647), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions.

Blank control: Raji cells (blue). Primary Antibody: Rabbit Anti-CD21 antibody (orb7143), dilution: 1 µg in 100 1 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Raji (Human) Cell Lysate at 30 ug, Primary: Anti-CD21 (orb7143) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 111 kD, Observed band size: 111 kD.

Quick Database Links

Gene Symbol

CR2

UniProt

P20023

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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