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CST3 Antibody
SKU: orb1335109
Description
Images & Validation
−Item 1 of 12
| Tested Applications | IHC, WB |
|---|---|
| Dilution range | WB 1:2000, IHC 1:150 |
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Full length human recombinant protein of human CST3(NP_000090) produced in HEK293T cell. |
| Target | CST3 |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
| Disclaimer | For research use only |
Alternative Names
−ARMD11; HEL-S-2
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Western blot analysis of extracts (35ug) from human plasma by using anti-CST3 monoclonal antibody (1:500).
Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-CST3 monoclonal antibody (1:500).
Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-CST3 monoclonal antibody (1:200).
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)(1:150)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CST3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min) (1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CST3 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CST3.
Quick Database Links
Gene Symbol
CST3
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
CST3 Antibody (orb1335109)
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