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CCL27 Antibody
SKU: orb1272644
Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits immunized with highly pure recombinant Human CTACK. Human CTACK specific antibody was purified by affinity chromatography employing an immobilized Human CTACK matrix. |
| Target | CCL27 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−ALP, ILC, CTAK, CTACK, PESKY, ESKINE, SCYA27
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To detect Human CTACK by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CTACK (orb1272633) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human CTACK.
To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human CTACK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human CTACK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained HeLa cells. The primary antibody was incubated at 3.0 ug/ml overnight at 4 °C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CCL27 Antibody (orb1272644)
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