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Epigenetics & Chromatin Biology
CTCF Antibodies for ChIP, WB, IHC and IF
High-quality, validated antibodies for analyzing chromatin architecture, transcriptional insulation, and 3D genome organization.
CTCF (CCCTC-binding factor) is a highly conserved zinc finger protein that acts as a master regulator of chromatin architecture and gene expression. As a critical protein in epigenetics and 3D genome structure, accurate detection of CTCF is essential for reproducible results in chromatin and gene regulation studies.
CTCF plays a central role in shaping the 3D genome by forming chromatin loops, defining topologically associating domains (TADs), and regulating enhancer-promoter interactions. Working closely with the cohesin complex, CTCF organizes genome architecture while exhibiting cell-type-specific binding patterns that influence distinct transcriptional programs. These dynamic functions make it a widely studied target in ChIP profiling, development, and cancer biology.
1. What Are CTCF Antibodies Used For?
High-affinity CTCF antibodies are deployed across a broad range of molecular biology and epigenetic applications:
- Chromatin immunoprecipitation (ChIP / ChIP-seq)
- Western blot (WB)
- Immunohistochemistry (IHC)
- Immunofluorescence (IF)
- Immunoprecipitation (IP)
- Flow cytometry (FC)
2. CTCF Antibodies for ChIP: Key Requirements
CTCF is one of the most frequently analyzed proteins in ChIP and ChIP-seq workflows. Given the complexity of chromatin immunoprecipitation, antibody performance dictates assay success.
Essential ChIP Antibody Attributes
- Strong, specific enrichment at known CTCF binding motifs.
- High signal-to-noise ratio over genomic background.
- Minimal non-specific cross-reactivity.
- High performance and epitope recognition within crosslinked chromatin structures.
Recommended ChIP-Validated Antibodies:
3. Knockout (KO) Validation & Specificity
Knockout (KO) validation is the gold standard for confirming antibody specificity, particularly in Western blotting and quantitative imaging applications.
Why KO Validation Matters
Validating an antibody against a genetically null background confirms absolute loss of signal in CTCF knockout samples, radically reducing the risk of non-specific binding and improving confidence in experimental data.
When to Prioritize KO-Validated Clones
Genetic validation is especially critical when conducting quantitative Western blots, precision immunocytochemistry (ICC/IF), or comparative quantitative protein analysis across distinct cell lines.
Recommended KO-Validated Antibody:
4. Formatting Options: Mouse vs. Rabbit Antibodies
Choosing between mouse monoclonal and rabbit-derived formats depends on your specific experimental design and multiplexing requirements:
Mouse Monoclonal
- Recognize a single defined epitope for high specificity.
- Reduce background in selected assays.
- Highly useful for multiplex co-staining experiments with rabbit primary antibodies.
Rabbit Polyclonal
- Recognize multiple epitopes, providing robust signal amplification.
- Consistently strong performance in ChIP and WB applications.
- Ideal for detecting low-abundance targets.
Recombinant Rabbit Monoclonal
- Combines single-epitope specificity with permanent sequence reproducibility.
- Eliminates batch-to-batch variation.
- The superior choice for long-term quantitative and longitudinal studies.
5. Recommended CTCF Antibodies by Application
Review Biorbyt's top-performing CTCF antibodies tailored for diverse molecular workflows:
| SKU | Product Name | Validated Applications | Size | Price |
|---|---|---|---|---|
| orb1260507 | Most popular CTCF Rabbit polyclonal antibody | ChIP, IF, IHC, IP, WB | 100 μl | $830 |
| orb1261271 | ChIP validated CTCF antibody | ChIP, IF, IHC, IP, WB | 100 μl | $830 |
| orb340732 | CTCF Rabbit polyclonal antibody | ChIP, IF, IHC, WB | 30 μl | $130 |
| orb1951174 | CTCF Rabbit monoclonal antibody (KD validated) | FC, ICC, IHC, WB | 50 μl | $260 |
| orb1519896 | Recombinant CTCF Rabbit monoclonal antibody | FC, ICC, IHC, IP, WB | 20 μl | $250 |
| orb2621233 | CTCF Rabbit polyclonal antibody (PE) | FC | 100 μg | $910 |
Why Choose Biorbyt CTCF Antibodies?
Biorbyt provides a comprehensive portfolio of CTCF antibodies for chromatin biology, validated extensively across ChIP, WB, IHC, IF, IP, and FC. From high-affinity polyclonals to Knockout-validated recombinant monoclonals, we supply the exact tools needed for reproducible genome organization research.
Search All CTCF Antibodies