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CTSA Antibody (N-term)
SKU: orb1938960
Description
Research Area
Cell Biology, Metabolism Research, Signal Transduction
Images & Validation
−Item 1 of 6
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | IHC-P-Leica - 1:500, WB - 1:2000 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 54466 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−PPGB
Quality Guarantee
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Anti-CTSA Antibody (N-term) at 1:2000 dilution + 293 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
CTSA Antibody (N-term) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the CTSA antibody detected the CTSA protein (arrow).
CTSA Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35 ug/lane). This demonstrates the CTSA antibody detected the CTSA protein (arrow).
Immunohistochemical analysis of paraffin-embedded Human kidney tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.
Immunohistochemical analysis of paraffin-embedded Human testis tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.
Western blot analysis of lysates from A431, Hela, HepG2, HT-1080 cell line and human placenta, kidney tissue lysate (from left to right), using CTSA Antibody (N-term). diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary Antibody. Lysates at 35 ug per lane.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
CTSA Antibody (N-term) (orb1938960)
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