Cyclin D1 Antibody PE Conjugate

SKU: orb248184

Description

Mouse monoclonal antibody against Cyclin D1 conjugated to PE

Images & Validation

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Item 1 of 2

Tested Applications	FACS, IF
Dilution Range	Flow Cytometry: 5ul/test/million cells or 5ul/test/100ul of whole blood,Immunofluorescence: 1:50-1:100 for 30 minutes at RT
Reactivity	Human, Monkey, Mouse, Rat
Application Notes	The dilution stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DCS-6 mAb to be titered up or down for optimal performance.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2a, kappa
Clone No.	DCS-6
Immunogen	Human recombinant full length Cyclin D1 protein was used as the immunogen.
Purification	Protein G affinity chromatography
Conjugation	PE

Storage & Handling

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Storage	Store the Cyclin D1 antibody at 2-8°C. Conjugate is light sensitive, store in the dark.
Buffer/Preservatives	500 ul at 0.1 mg/ml with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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FACS testing of Jurkat cells: Black = cells alone; Green = isotype control; Red = Cyclin D1 antibody

FACS testing of MCF-7 cells: Black = cells alone; Green = isotype control; Red = Cyclin D1 antibody

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Protocol Information

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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