Cyclin D1 Recombinant Rabbit Monoclonal Antibody

SKU: orb559087

Description

Research Area

Cell Biology, Cyclin D family

Images & Validation

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Item 1 of 2

Tested Applications	ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	2H4
Immunogen	A synthesized peptide derived from human Cyclin D1 (261-295aa)
Target	CCND1
Molecular Weight	32 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CyclinD1; Cyclin-D1; B cell ccl/lymphoma 1; B cell leukemia 1; B-cell CLL/lymphoma 1; B-cell leukemia 1; B-cell lymphoma 1 protein; BCL-1; BCL1; BCL1 oncogene; CCND 1; CCND1; CCND1 protein; CCND1/FSTL3 fusion gene, included; CCND1/IGHG1 fusion gene; CCND1/IGHG1 fusion gene, included; CCND1/IGLC1 fusion gene, included; CCND1/PTH fusion gene, included; Parathyroid adenomatosis 1; PRAD1; FSTL3; CCND1_HUMAN; AI327039; B cell lymphoma 1 protein; BCL 1; BCL-1; BCL-1 oncogene; BCL1 oncogene; CCND1/FSTL3 fusion gene, included; cD1; Cyl 1; D11S287E; G1/S specific cyclin D1; G1/S-specific cyclin-D1.

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Cyclin D1 Recombinant Rabbit Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cyclin D1) Monoclonal Antibody, Unconjugated (orb559087) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Lane 3: A549 (Human) Cell Lysate at 30 ug, Lane 4: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-Cyclin D1 (orb559087) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.