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CYP2E1 Recombinant Rabbit Monoclonal Antibody
SKU: orb704324
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Description
Research Area
Alcohol Metabolism, Cancer Research, Drug Metabolism, Metabolism
Images & Validation
−Item 1 of 6
| Tested Applications | ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 1A26 |
| Immunogen | KLH conjugated synthetic peptide derived from human CYP2E1 |
| Target | CYP2E1 |
| Molecular Weight | 57 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
Alternative Names
−4-nitrophenol 2-hydroxylase; CP2E1_HUMAN; CPE 1; CPE1; CYP2E; CYP2E1; CYP2E1; CYPIIE1; Cytochrome P450 2E1; cytochrome P450 family 2 subfamily E polypeptide 1; Cytochrome P450 isozyme 3A; Cytochrome P450 LM3A; Cytochrome P450 subfamily IIE(ethanol inducible) polypeptide 1; Cytochrome P450, family 2, subfamily e, polypeptide 1; cytochrome P450, subfamily IIE(ethanol-inducible), polypeptide 1; Cytochrome P450, subfamily IIE; Cytochrome P450-ALC; Cytochrome P450-J; Cytochrome P450RLM6; EC 1.14.13.-; EC 1.14.13.n7; Ethanol inducible p450; Ethanol inducible p450; Ethanol-inducible P450; flavoprotein-linked monooxygenase; MGC133529; microsomal monooxygenase; OTTHUMP00000020813; OTTHUMP00000046571; P450 ALC; P450 J; P450-J; P450C2E; P450C2E; P450J; P450RLM6; xenobiotic monooxygenase.
Quality Guarantee
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293T cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CYP2E1) monoclonal Antibody, Unconjugated (orb704324) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CYP2E1) monoclonal Antibody, Unconjugated (orb704324) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CYP2E1) Monoclonal Antibody, Unconjugated (orb704324) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CYP2E1) Monoclonal Antibody, Unconjugated (orb704324) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CYP2E1) Monoclonal Antibody, Unconjugated (orb704324) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: human liver tissue lysates, Primary: Anti-CYP2E1 (orb704324) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.
Quick Database Links
Gene Symbol
CYP2E1
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ICC
Immunocytochemistry
CYP2E1 Recombinant Rabbit Monoclonal Antibody (orb704324)
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