Cytochrome C Rabbit Polyclonal Antibody

SKU: orb221350

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Description

Research Area

Apoptosis, Cell Biology, Cell Cycle, Cytochrome c, Energy Metabolism, Metabolism, Mitochondrial, Mitochondrial Markers, Transcriptional Regulators

Images & Validation

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Item 1 of 4

Tested Applications	ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, ICC/IF=1:100-500, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Bovine, Equine, Porcine

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Cytochrome C (51-105/105aa)
Target	CYCS
Molecular Weight	14.4 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CytC; CYC; CYCS; Cytochrome c somatic; HCS; CYC_HUMAN; Cytochrome-c; MSA06; THC4.

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Blank control: HepG2 (blue). Primary Antibody: Rabbit Anti-Cytochrome C antibody (orb221350, Green), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde for 10 min at 37°C. Primary antibody (orb221350, 1 µg/1x10^6 cells) were incubated for 30 min at room temperature, followed by 1X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Cytochrome C protein at 30 ug, Primary: Anti-Cytochrome C (orb221350) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14 kD, Observed band size: 14 kD.

Sample: Lane 1: Heart (Rat) Lysate at 40 ug, Lane 2: Heart (Mouse) Lysate at 40 ug, Primary: Anti-Cytochrome C (orb221350) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14.4 kD, Observed band size: 14.4 kD.

Tissue/Cell: Sh-sy5y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Cytochrome C) polyclonal Antibody, Unconjugated (orb221350) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

CYCS

UniProt

P99999

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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ICC

Immunocytochemistry

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