Cytokeratin 1 Rabbit Polyclonal Antibody (PE)

SKU: orb485921

Description

Cytokeratin 1 Rabbit Polyclonal Antibody (PE) is a PE conjugated antibody targeting KRT1. This antibody is suitable for FC, IF. It exhibits reactivity with Human, Mouse, Rabbit samples.

Research Area

Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications	FC, IF
Dilution Range	Flow-Cyt=1μg/Test, IF=1:100-500
Reactivity	Human, Mouse, Rabbit
Predicted Reactivity	Bovine, Canine, Equine, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Cytokeratin 1 (301-400/644aa)
Target	KRT1
Purification	Affinity purified by Protein A
Conjugation	PE

Storage & Handling

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Storage	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Cytokeratin-1; 67 kDa cytokeratin; CK 1; CK1; Cytokeratin1; EHK1; Hair alpha protein; K 1; K1; Keratin 1; Keratin type II cytoskeletal 1; Keratin1; KRT 1; KRT1A; K2C1_HUMAN; Keratin, type II cytoskeletal 1; Cytokeratin-1; CK-1; Keratin-1; Type-II keratin Kb1.

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Cytokeratin 1 Rabbit Polyclonal Antibody (PE)

Blank control (blue line): Hela cells (blue). Primary Antibody (green line): Rabbit Anti-Cytokeratin 1/FITC Conjugated antibody (orb485921), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-PE. Protocol, The cells were fixed with 70% ice-cold methanol overnight at 4°C. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

KRT1

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Datasheet

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Protocol Information

Flow Cytometry

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Immunofluorescence

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