Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody

SKU: orb559083

Featured

Description

Research Area

Signal Transduction

Images & Validation

−

Item 1 of 4

Tested Applications	ICC, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Mouse, Rat

Related Conjugates & Formulations

−

FITC

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	IgG
Clone No.	1C2
Immunogen	A synthesized peptide derived from human Cytokeratin 1 (600-644aa)
Target	KRT1
Source/Expression System	293F
Molecular Weight	70 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Cytokeratin-1; 67 kDa cytokeratin; CK 1; CK1; Cytokeratin1; EHK1; Hair alpha protein; K 1; K1; Keratin 1; Keratin type II cytoskeletal 1; Keratin1; KRT 1; KRT1A; K2C1_HUMAN; Keratin, type II cytoskeletal 1; Cytokeratin-1; CK-1; Keratin-1; Type-II keratin Kb1.

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

25 ug total protein per lane of various lysates probed with Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody (orb559083) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Mouse Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody (orb559083) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L, HRP conjugated (orb572747) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody (orb559083) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L, HRP conjugated (orb572747) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody (orb559083) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L, HRP conjugated (orb572747) and DAB staining.

Quick Database Links

Gene Symbol

KRT1

UniProt

P04264

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

IHC-Fr

Immunohistochemistry Frozen

View Protocol

Immunofluorescence

View Protocol

ICC

Immunocytochemistry

View Protocol

Find More Protocols