Recombinant Cytokeratin 18 Antibody

SKU: orb2642178

Description

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Images & Validation

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Tested Applications	FACS, IF, IHC-P, WB
Dilution Range	Flow cytometry: 1-2ug/10^6 cells,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the recombinant Cytokeratin 18 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	KRT18/2819R
Immunogen	Full length recombinant human protein was used as the immunogen for the recombinant Cytokeratin 18 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Immunofluorescent staining of permeabilized human MCF7 cells with recombinant Cytokeratin 18 antibody (clone KRT18/2819R, blue) and Reddot nuclear stain (red).

IHC staining of FFPE human colon carinoma with recombinant Cytokeratin 18 antibody (clone KRT18/2819R). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human HCT116 antibody with recombinant Cytokeratin 18 antibody (clone KRT18/2819R). Predicted molecular weight ~48 kDa.

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Cytokeratin 18 antibody (clone KRT18/2819R); Red = isotype control, Blue = recombinant Cytokeratin 18 antibody.

SDS-PAGE analysis of purified, BSA-free recombinant Cytokeratin 18 antibody (clone KRT18/2819R) as confirmation of integrity and purity.

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UniProt

P05783

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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