Recombinant Keratin 18 Antibody / Cytokeratin 18

SKU: orb2642424

Description

Keratin 18 (KRT18) is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. [Wiki]

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Tested Applications	FACS, IHC-P, WB
Dilution Range	Flow cytometry: 1-2ug/10^6 cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Reactivity	Human
Application Notes	The stated application concentrations are suggested starting points. Titration of the recombinant Keratin 18 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	CTKN18-2R
Immunogen	Human full length recombinant protein was used as the immunogen for the recombinant Keratin 18 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant Keratin 18 Antibody / Cytokeratin 18

Western blot testing of human HCT116 antibody with recombinant Keratin 18 antibody (clone CTKN18-2R). Predicted molecular weight ~48 kDa.

IHC staining of FFPE human colon carinoma with recombinant Keratin 18 antibody (clone CTKN18-2R). Required HIER: boil tissue sections in 10 mM citrate buffer, pH6, for 10-20 min and allow to cool before testing.

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Keratin 18 antibody (clone CTKN18-2R); Red = isotype control, Blue = recombinant Keratin 18 antibody.

Quick Database Links

UniProt

P05783

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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