Recombinant CK18 Antibody / Cytokeratin 18

SKU: orb2642516

Description

This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, also called KRT18 and CK18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Images & Validation

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Tested Applications	IHC-P
Dilution Range	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Reactivity	Human
Application Notes	Optimal dilution of the recombinant CK18 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	KRT18/2808R
Immunogen	Full length recombinant human protein was used as the immunogen for the recombinant CK18 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant CK18 Antibody / Cytokeratin 18

IHC staining of FFPE human colon carinoma with recombinant CK18 antibody (clone KRT18/2808R). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human thyroid carinoma with recombinant CK18 antibody (clone KRT18/2808R). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant CK18 antibody (clone KRT18/2808R) as confirmation of integrity and purity.

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UniProt

P05783

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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