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Cytokeratin 20, CK20 Antibody

SKU: orb1294302

Description

Rabbit polyclonal antibody to Cytokeratin 20

Images & Validation

Tested ApplicationsIF, IHC, WB
Dilution rangeWestern Blot 1:1000–1:2000 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:200–1:300
ReactivityHuman

Key Properties

HostRabbit
ClonalityPolyclonal
IsotypeRabbit-IgG
ImmunogenSynthetic peptide / encompassing a sequence within the center region
TargetCytokeratin 20, CK20
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
DisclaimerFor research use only

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Cytokeratin 20, CK20 Antibody

Anti-Cytokeratin 20 antibody - at 1/2000 dilution, Lysates/proteins at 45 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with orb1294302 overnight at 4°C.

Cytokeratin 20, CK20 Antibody

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294302 anti-Cytokeratin 20 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Cytokeratin 20, CK20 Antibody

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294302 anti-Cytokeratin 20 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Cytokeratin 20, CK20 Antibody

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 20 with orb1294302 at 1/200.

Cytokeratin 20, CK20 Antibody

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 20 with orb1294302 at 1/200.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol

Cytokeratin 20, CK20 Antibody (orb1294302)

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25 μl
$ 260.00
100 μl
$ 390.00
DispatchUsually dispatched within 5-10 working days
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