Cytokeratin 5/6 Antibody

SKU: orb1825706

Description

Cytokeratin 5 is a basic protein that is closely related to Cytokeratin 6. They share similar tissue distribution and are found in various proportions in many non-keratinizing stratified squamous epithelia eg. tongue mucosa, as well as in basal epithelia of trachea, basal cells of epidermis, hair follicles, sebaceous and sweat glands of skin, luminal cells of the mammary gland, basal cells of prostate, urothelium, vagina and endocervical mucosa. Cytokeratins 5 and 6 are also expressed in basal cell epitheliomas, squamous cell carcinomas of skin, tongue, epiglottis and of the rectal-anal region. Cytokeratin 5/6 mAb is a useful aid for classification of epithelioid mesotheliomas.

Images & Validation

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Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the Cytokeratin 5/6 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	KRT5.6/8275R
Immunogen	Human Cytokeratin 5- and Cytokeratin 6-homologous recombinant protein fragments were used as the immunogen for the Cytokeratin 5/6 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC staining of FFPE human prostate carcinoma tissue with Cytokeratin 5/6 antibody (clone KRT5.6/8275R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Negative control: IHC testing of FFPE human brain tissue with Cytokeratin 5/6 antibody (clone KRT5.6/8275R) at 2 ug/ml. HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P02538, P13647

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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