You have no items in your shopping cart.
Cytokeratin 5 Rabbit Polyclonal Antibody
SKU: orb1294308
Description
Research Area
Cancer Biology
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:1000–1:2000, Immunofluorescence 1:200–1:500, Immunohistochemistry (Paraffin) 1:100–1:300 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the center region |
| Target | Cytokeratin 5/6 |
| Molecular Weight | 43-55 kDa |
| Purity | Affinity column purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Concentration | 1.74 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Similar Products
−- Item 1 of 15
Pan Cytokeratin Rabbit Polyclonal Antibody [orb10399]
FC, ICC, IF, IHC-Fr, IHC-P, WB
Bovine, Canine, Equine, Gallus, Porcine, Rabbit
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl, 200 μl - Item 1 of 4
Cytokeratin 5 Rabbit Polyclonal Antibody [orb10408]
IF, IHC-Fr, IHC-P, WB
Bovine, Canine, Equine, Porcine, Rabbit
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl, 200 μl - Item 1 of 4
Cytokeratin 5 rabbit pAb Antibody [orb765008]
ELISA, IF, IHC, WB
Human, Mouse, Rat
Polyclonal
Unconjugated
100 μl, 50 μl - Item 1 of 5
Cytokeratin 4/KRT4 Rabbit Polyclonal Antibody [orb1676537]
ELISA, IF, IHC, WB
Human
Rabbit
Polyclonal
Unconjugated
100 μg - Item 1 of 3
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.
All lanes: Anti-Cytokeratin 5/6 antibody - at 1/2000 dilution, Lysates/proteins at 45 µg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294308 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294308 anti-Cytokeratin5/6 antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294308 anti-Cytokeratin5/6 antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 5/6 with orb1294308 at 1/100.
Quick Database Links
Gene Symbol
Cytokeratin 5/6
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
Cytokeratin 5 Rabbit Polyclonal Antibody (orb1294308)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days