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Cytokeratin 5/8 Antibody
SKU: orb2639591
Description
Images & Validation
−Item 1 of 3
| Tested Applications | FACS, IF, IHC-P, WB |
|---|---|
| Dilution Range | Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1, kappa |
| Clone No. | C-50 |
| Immunogen | A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody. |
| Purification | Protein G affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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IHC analysis of formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5/8 antibody (clone C-50). Required HIER: digest sections with Trypsin at 1mg/ml, 15 min, at RT.
Western blot testing of HeLa cell lysate and Cytokeratin 5/8 antibody (clone C-50). Predicted molecular weight: 52-58kDa but observed here at ~43kDa.
IHC analysis of formalin-fixed, paraffin-embedded human colon carcinoma stained with Cytokeratin 5/8 antibody (clone C-50). Required HIER: digest sections with Trypsin at 1mg/ml, 15 min, at RT.
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
IF
Immunofluorescence
Cytokeratin 5/8 Antibody (orb2639591)
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