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Danoprevir

SKU: orb1689756

Description

Danoprevir (RG7227) is a potent, peptidomimetic inhibitor of the HCV NS3/4A protease, exhibiting activity in the low nanomolar range (IC50=0.2-3.5 nM). It has been utilized in both in vitro enzymatic assays and in vivo animal studies to investigate hepatitis C virus replication and protease function.

Research Area

Infectious Disease & Virology, Protein Biochemistry

Images & Validation

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Danoprevir

Key Properties

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CAS Number850876-88-9
MW731.83
Purity99.80%
FormulaC35H46FN5O9S
SMILESCC(C)(C)OC(=O)N[C@H]1CCCCC\C=C/[C@H]2C[C@]2(NC(=O)[C@@H]2C[C@H](CN2C1=O)OC(=O)N1Cc2cccc(F)c2C1)C(=O)NS(=O)(=O)C1CC1
TargetSARS-CoV,HCV Protease
SolubilityH2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:150 mg/mL (204.97 mM);Ethanol:133 mg/mL (181.74 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (5.47 mM)

Bioactivity

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Target IC50
HCV NS3/4A protease:0.2 nM-3.5 nM
In Vivo
In rats or monkeys,Danoprevir (30 mg/kg) was administered.
Cell Research
Serially diluted Danoprevir is added to Huh7 cells harboring the K2040 replicon 1 day after cell plating. For antiviral assays, after a 48-hour incubation, intracellular RNA is extracted, and the level of HCV replicon RNA is quantified by reverse transcription (RT)-PCR assay with the primers (5'-CACTCCCCTGTGAGGAACTACTG-3' and 5'-AGGCTGCACGACACTCATACT-3') and a probe (5'-6-FAM-CTTCACGCAGAAAGCGTCTAGCCATGG-MGBNFQ-3' using an ABI Prism 7900 sequence detection system. Here, FAM is 6-carboxyfluorescin and MGBNFQ is a molecular-groove binding non-fluorescence quencher specific to the HCV 5' untranslated region. Single-tube reactions are performed using the TaqMan Gold RT-PCR kit. Triplicate reactions for the RNA standards and samples are performed in 50 μL with 5 μL intracellular RNA (50 ng). RT is carried out at 48 °C for 30 min followed by 10 min at 95 °C. The PCR is run as follows: 15 seconds at 95 °C and 1 min at 60 °C for 40 cycles. Each RNA concentration is determined in triplicate. The absolute concentration of replicon RNA is calculated based on its signal relative to that of a standard curve generated by known concentrations of an in vitro-transcribed RNA corresponding to a genotype 1b 5' untranslated region. Replicon levels in the presence of Danoprevir are fitted to a four-parameter logistic function to obtain EC50.(Only for Reference)
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Storage & Handling

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Storagestore at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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SARS-CoV, SARSCoV, SARS coronavirus, RO 5190591, RO5190591, RO-5190591, RG7227, RG-7227, RG 7227, R 7227, R7227, R-7227, HCV NS3/4A protease, HCV Protease, HCVProtease, HCV, Hepatitis C virus, Inhibitor, inhibit, ITMN-191, ITMN 191, ITMN191, Danoprevir

Similar Products

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  • Danoprevir

    Danoprevir [orb1223857]

    >98% (HPLC)

    850876-88-9

    731.8

    C35H46FN5O9S

    1 g, 500 mg, 200 mg, 100 mg, 10 mg, 5 mg, 25 mg, 50 mg
Quality Guarantee

Quality Guarantee

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Compound Identifiers

PubChem CID
25144717 ↗

Key Properties

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No computed properties available.

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Danoprevir (orb1689756)

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Step 1: Enter information below

(Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

Select a size below

1 mg
$ 100.00
5 mg
$ 180.00
1 ml x 10 mM (in DMSO)
$ 270.00
10 mg
$ 290.00
25 mg
$ 470.00
50 mg
$ 670.00
100 mg
$ 930.00
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