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DDB2 Antibody
SKU: orb1258426
Featured
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 3
| Tested Applications | IF, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-315 of human DDB2 (NP_000098.1). |
| Target | DDB2 |
| Molecular Weight | Observed: 48kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DDBB, UV-DDB2, XPE, DNA damage-binding protein 2, DDB p48 subunit, UV-DDB 2, UV-damaged DNA-binding protein 2, damage-specific DNA binding protein 2, 48kDa, xeroderma pigmentosum group E protein
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Western blot analysis of extracts of various cell lines, using DDB2 antibody (orb1258426) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of MCF-7 cells using DDB2 antibody (orb1258426). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using DDB2 antibody (orb1258426). Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.
Quick Database Links
Gene Symbol
DDB2
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
DDB2 Antibody (orb1258426)
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