DEFB1/BD-1 Antibody

SKU: orb1539002

Description

DEFB1/BD-1 Antibody

Images & Validation

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Tested Applications	ELISA, IHC, IHC-P, WB
Dilution Range	ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml)
Reactivity	Human
Application Notes	Resuspension: Reconstitute with sterile water to 0.1-1.0 mg/ml.. Further information: This in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. ELISA: To detect hBD-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BD-1 as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBD-1. Western Blot: To detect hBD-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. Read more...

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	E.coli derived recombinant Human BD-1.
Target	DEFB1 / BD-1
Purification	Immunoaffinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Lyophilized from PBS, pH 7.2
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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DEFB1, BD1, Beta-defensin 1, BD-1, Beta Defensin 1, DEFB-1, DEFB101, Defensin, beta 1, Defensin beta, HBD-1, Beta-defensin-1, HBD1

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Quick Database Links

Gene Symbol

DEFB1 / BD-1

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Datasheet

Product Information

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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