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DERL1 Antibody
SKU: orb1262529
Featured
Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This DERL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human DERL1. |
| Target | DERL1 |
| Molecular Weight | 29 kDa |
| Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Derlin-1, Degradation in endoplasmic reticulum protein 1, DERtrin-1, Der1-like protein 1, DERL1, DER1
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Western Blot at 1:16000 dilution + SK-BR-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of DERL1 Antibody in mouse NIH-3T3 cell line lysates (35 ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with DERL1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
DERL1 Antibody (orb1262529)
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