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Desmin Mouse Monoclonal Antibody
SKU: orb1294251
Description
Research Area
Cardiovascular Research
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:500-1:1000, Immunofluorescence 1:100-1:200, Immunohistochemistry (Paraffin) 1:50-1:100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a |
| Target | Desmin |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Anti-Desmin antibody at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 10% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294251 overnight at 4°C.
Anti-Desmin antibody at 1/1000 dilution, Lysates/proteins at 50 µg per lane. This blot was produced using a 10% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294251 overnight at 4°C. Troponin I, cardiac muscle (orb1294291). Troponin T, cardiac muscle (orb1294292).
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294251 anti-Desmin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Desmin monoclonal antibody orb1294251. Immunoperoxidase staining of formalin fixed, paraffin-embedded human smooth muscle tissue.
Quick Database Links
Gene Symbol
Desmin
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
Desmin Mouse Monoclonal Antibody (orb1294251)
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