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Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution Range | IF - 1:10-50, WB - 1:1000, IHC-P-Leica - 1:500 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | BTD53579 |
| Immunogen | This DLL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 545-573 amino acids from the C-terminal region of human DLL3. Antigen Region: 545-573 aa. |
| Target | DLL3 |
| Molecular Weight | 64618 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Delta-like protein 3, Drosophila Delta homolog 3, Delta3, DLL3
Similar Products
−- Item 1 of 7
DLL3 Antibody (C-term) [orb1927976]
IF, IHC-P, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
200 μl
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All lanes: Anti-DLL3 Antibody (C-term) at 1:1000 dilution. Lane 1: TT whole cell lysate. Lane 2: Human brain lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-DLL3 Antibody (C-term) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: A549 whole cell lysate. Lane 3: Human brain lysate. Lane 4: SH-SY5Y whole cell lysate. Lane 5: U-2OS whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of DLL3 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
DLL3 Antibody (C-term) western blot analysis in 293 cell line lysates (35 ug/lane). This demonstrates the DLL3 antibody detected the DLL3 protein (arrow).
Immunohistochemical analysis of paraffin-embedded Human brain tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_058637.1, NP_982353.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
DLL3 Antibody (C-term) (orb1937511)
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