λDNA/ EcoRI Digest

SKU: orb1734267

Description

The EcoRI digest of λ DNA yields the following 6 discrete fragments (in base pairs): 21226*, 7421, 5804, 5643, 4878, 3530*. The cohesive ends (12b cos site of bacteriophage lambda) of fragments 21226 bp and 3530 bp may anneal and form an additional band. These fragments may be separated again by heating to 65 °C for 5 min and then cooling on ice for 3 min.

Images & Validation

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Application Notes	Preparation: Lambda DNA (48.502 bp) was completely digested by EcoRI, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Recommended Load: 2-5 µl per lane.

Key Properties

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Storage & Handling

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Storage	store at -20 °C. Short term storage (up to 1 month) at 4 °C possible
Form/Appearance	liquid (supplied in 6x Green Gel Loading Buffer)
Concentration	50 ng/µl
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ready-to-use

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