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DNAJA1 Antibody
SKU: orb1264047
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Bovine, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This DNAJA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the Central region of human DNAJA1. |
| Target | DNAJA1 |
| Molecular Weight | 45 kDa |
| Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−DnaJ homolog subfamily A member 1, DnaJ protein homolog 2, HSDJ, Heat shock 40 kDa protein 4, Heat shock protein J2, HSJ-2, Human DnaJ protein 2, hDj-2, DNAJA1, DNAJ2, HDJ2, HSJ2, HSPF4
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Western blot analysis in MCF-7 cell line lysates (35 ug/lane).
DNAJA1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of DNAJA1 using rabbit polyclonal DNAJA1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DNAJA1 gene.
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
DNAJA1 Antibody (orb1264047)
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