DNAJC19 Antibody

SKU: orb1257603

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Description

DNAJC19 Antibody

Images & Validation

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Item 1 of 4

Tested Applications	IF, IHC, WB
Reactivity	Human, Mouse, Rat
Application Notes	WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human DNAJC19 (NP_660304.1).
Target	DNAJC19
Molecular Weight	Observed: 12kDa
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid
Concentration	batch dependent
Disclaimer	For research use only

Alternative Names

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Mitochondrial import inner membrane translocase subunit TIM14, DnaJ homolog subfamily C member 19, DNAJC19, TIM14, TIMM14

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Western blot analysis of extracts of various cell lines, using DNAJC19 antibody (orb1257603) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunohistochemistry of paraffin-embedded human colon carcinoma using DNAJC19 antibody (orb1257603) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using DNAJC19 antibody (orb1257603) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of Hela cells using DNAJC19 antibody (orb1257603) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

DNAJC19

UniProt

Q96DA6

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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