Dog IgG F(ab')2 Antibody Peroxidase Conjugated

SKU: orb346587

Description

Dog IgG F(ab')2 antibody (Peroxidase)

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:500 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Reactivity	Canine
Application Notes	Anti-Dog IgG F(ab’)2 Peroxidase Conjugate has been tested by ELISA. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. 0.43 moles Peroxidase (HRP) per mole. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 2.0 mL

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Dog IgG F(ab')2 fragment
Purity	This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Dog IgG, Dog IgG F(ab’)2 and Dog Serum. No reaction was observed against Dog IgG F(c).
Conjugation	HRP

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration	10.0 mg/mL
Disclaimer	For research use only

Alternative Names

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rabbit anti-Dog IgG F(ab')2 Antibody Peroxidase Conjugated, rabbit anti-Dog IgG Fab2 HRP Conjugated Antibody

Dog IgG F(ab')2 Peroxidase Antibody [orb346089]

Conjugation:	HRP
Purity:	This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Dog IgG, anti-Dog IgG F(ab’)2 and anti-Dog Serum. No reaction was observed against anti-Dog IgG F(c) or anti-Pepsin.
Source:	Dog

Sizes Available: 1 mg

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Dog IgG F(ab')2 Antibody Peroxidase Conjugated

ELISA results of purified Rabbit Anti-Dog IgG F(ab')2 Peroxidase Antibody tested against purified Dog IgG Fab2. Each well was coated in duplicate with 1.0 µg of Dog IgG F(ab')2 (red line). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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