Dog Surfactant Associated Protein A2 (SPA2) ELISA Kit

SKU: orb1291286

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Description

Dog SPA2 (Surfactant Associated Protein A2) ELISA Kit

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog SPA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog SPA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog SPA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog SPA2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Canine
Sample Types	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.059 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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SFTPA2B, COLEC5, SP2A, SPA1 beta, SP-A2, SPAII, Collectin-5, Pulmonary Surfactant Protein A2, 35 kDa pulmonary surfactant-associated protein, Alveolar proteinosis protein

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Dog Surfactant Associated Protein A2 (SPA2) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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