Recombinant TMEM16A Antibody / DOG1 / ANO1

SKU: orb2642573

Description

Expression of DOG1/ANO1/TMEM16A protein is elevated in the gastrointestinal stromal tumors (GIST's), c-kit signaling-driven mesenchymal tumors of the GI tract. TMEM16A is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for TMEM16A has been reported in 97.8 percent of scorable GIST's, including all c-kit negative GIST's. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of TMEM16A and c-kit in GIST's is nearly identical: 94.4% vs. 94.7%.

Images & Validation

−

Item 1 of 2

Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant TMEM16A antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	DG1/2831R
Immunogen	A portion of amino acids 2-101 from the human protein was used as the immunogen for this recombinant TMEM16A antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Recombinant TMEM16A Antibody / DOG1 / ANO1

IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with TMEM16A antibody (clone DG1/2831R). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant TMEM16A antibody (clone DG1/2831R) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q5XXA6

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

IHC-P

Immunohistochemistry Paraffin

View Protocol

Find More Protocols