Donkey anti-Sheep IgG (H&L), Biotin conjugated

SKU: orb699951

Description

Donkey polyclonal antibody against Donkey anti-Sheep IgG (H&L) conjugated to Biotin

Images & Validation

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Tested Applications	ELISA, IHC, WB
Dilution range	1 : 2000-1: 20 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 2000-1: 20 000 (WB)
Reactivity	Sheep
Application Notes	No reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Key Properties

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Host	Donkey
Clonality	Polyclonal
Immunogen	Purified sheep IgG, whole molecule
Purity	Immunogen purified donkey IgG.
Purification	Antibody concentration is 3.85 mg/ml and it has been afinity purified on solid phase sheep IgG (H&L)biotin conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. It contains 0.05 % sodium azide as preservative
Conjugation	Biotin

Storage & Handling

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Storage	Store non-diluted antibody at 2-8°C. Shelf life is 1 year from date of receipt.
Form/Appearance	Liquid
Disclaimer	For research use only

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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