Donkey IgG (H&L) Antibody

SKU: orb347992

Description

Donkey IgG (H&L) antibody

Images & Validation

−

Item 1 of 1

Tested Applications	ELISA, IHC, WB
Dilution range	ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Reactivity	Donkey
Application Notes	Anti-Donkey IgG antibody has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Key Properties

−

Antibody Type	Secondary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG
Immunogen	Donkey IgG whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Donkey IgG and Donkey Serum.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	2.0 mg/mL
Disclaimer	For research use only

Alternative Names

−

goat anti-Donkey IgG Antibody, goat anti Donkey IgG Antibody

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

ELISA Results of Goat Anti-Donkey IgG Antibody tested against purified Donkey IgG. Each well was coated in duplicate with 1.0 µg of Donkey IgG (p/n orb2652734). The working dilution is 1:38200. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Donkey Anti-Goat IgG HRP conjugated (p/n orb347032), and TMB substrate (p/n orb348651).

Quick Database Links

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols