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E 64c

SKU: orb1300647

Description

E-64c (EP 475) is a cell-permeable, irreversible inhibitor of cysteine proteases. This compound is widely used in biochemical research to study protease function in both cellular (in vitro) and animal (in vivo) models of diseases such as muscular dystrophy and cancer.

Research Area

Infectious Disease & Virology, Protein Biochemistry

Images & Validation

Key Properties

CAS Number76684-89-4
MW314.38
Purity99.90% (May vary between batches)
FormulaC15H26N2O5
SMILESCC(C)CCNC(=O)C(CC(C)C)NC(=O)C1OC1C(O)=O
TargetSARS-CoV,Cysteine Protease
SolubilityH2O:2 mg/mL (6.36 mM);DMSO:58 mg/mL (184.49 mM);Ethanol:58 mg/mL (184.49 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (6.36 mM)

Bioactivity

In Vivo
In pectoral muscle of dystrophic chickens, Loxistatin Acid (E-64C) normalizes the increased activities of cathepsin B and cathepsin H. E-64C inhibits ischemic degradation of cerebral proteins following occlusion of the middle cerebral artery in rats.
In Vitro
Loxistatin Acid (E-64C), as a thiol protease inhibitor, inhibits calpain activity in intact platelets. E-64C inhibits β-secretase activity in regulated secretory vesicles of neuronal chromaffin cells, and decrease Aβ production, indicating a therapeutic strategy for Alzheimer's disease.

Storage & Handling

StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

NSC694279, NSC-694279, Loxistatin Acid, NSC 694279, inhibit, Inhibitor, EP475, EP-475, EP 475, Cathepsin, E 64c, CysteineProtease, Cysteine Protease, Cysteine protease, SARS coronavirus, SARSCoV, SARS-CoV

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Quality Guarantee

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Key Properties

No computed properties available.

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Protocol Information

E 64c (orb1300647)

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(Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

Select a size below

1 mg
$ 70.00
1 ml x 10 mM (in DMSO)
$ 110.00
5 mg
$ 110.00
10 mg
$ 150.00
25 mg
$ 220.00
50 mg
$ 320.00
100 mg
$ 440.00
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