E-Cadherin Antibody / CDH1

SKU: orb2642021

Description

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. [UniProt]

Images & Validation

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Tested Applications	IF, IHC-P, WB
Dilution range	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 2-4ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the E-Cadherin antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1, kappa
Clone No.	ECD1-2
Immunogen	A full length recombinant human protein was used as the immunogen for the E-Cadherin antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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Immunofluorescent staining of human MCF7 cells with E-Cadherin antibody (clone ECD1-2, green) and Reddot nuclear stain (red).

IHC testing of FFPE human skin with E-Cadherin antibody (clone ECD1-2). HIER: steam sections in pH6 citrate buffer for 20 min.

IHC testing of FFPE human placenta with E-Cadherin antibody (clone ECD1-2). HIER: steam sections in pH6 citrate buffer for 20 min.

Western blot testing of 1) partial recombinant protein and 2) human stomach lysate using E-Cadherin antibody at 0.5 ug/ml. Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).

Quick Database Links

UniProt

P12830

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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