E-selectin | CD62E Rabbit Polyclonal Antibody

SKU: orb1294258

Description

Rabbit polyclonal antibody to E selectin

Research Area

Cardiovascular Research

Images & Validation

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Item 1 of 4

Tested Applications	IHC, WB
Dilution Range	Western Blot 1:500-1:1000, Immunofluorescence 1:200-1:500, Immunohistochemistry (Paraffin) 1:100-1:200
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide / Extracellular domain corresponding to Human E selectin / CD62E
Target	E-selectin \| CD62E
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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E-selectin | CD62E Rabbit Polyclonal Antibody

Anti E-selectin / CD62E antibody at 1/500 dilution. Lysates at 50 µg per lane, Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294258 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling E-selectin / CD62E with orb1294258 at 1/100.

Quick Database Links

Gene Symbol

E-selectin | CD62E

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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