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EDG-1 (phospho Thr236) rabbit pAb Antibody
SKU: orb767915
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Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IF, WB |
|---|---|
| Dilution range | Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human S1P Receptor EDG1 around the phosphorylation site of Thr236. AA range:206-255 |
| Molecular Weight | 42 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-S1PR1 antibody, anti-CHEDG1 antibody, anti-EDG1 antibody, anti-Sphingosine 1-phosphate receptor 1 antibody, anti-S1P receptor 1 antibody, anti-S1P1 antibody, anti-Endothelial differentiation G-protein coupled receptor 1 antibody, anti-Sphingosine 1-phosphate receptor Edg-1 antibody, anti-S1P receptor Edg-1 antibody, anti-CD antibody
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Western blot analysis of HEPG2-UV MOUSE-BRAIN using p-EDG-1 (T236) antibody. Antibody was diluted at 1:500.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using S1P Receptor EDG1 (Phospho-Thr236) Antibody.
Immunofluorescence analysis of COS7 cells, using S1P Receptor EDG1 (Phospho-Thr236) Antibody. The picture on the right is blocked with the phospho peptide.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
EDG-1 (phospho Thr236) rabbit pAb Antibody (orb767915)
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