EFEMP1 Antibody (N-term)

SKU: orb1928113

This product can be custom made

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Infectious Disease & Virology, Neuroscience, Signal Transduction

Images & Validation

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Item 1 of 4

Tested Applications	FC, IHC-P, WB
Dilution Range	WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50
Reactivity	Human, Mouse
Predicted Reactivity	Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	BTD44362
Immunogen	This EFEMP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the N-terminal region of human EFEMP1. Antigen Region: 119-148 aa.
Target	EFEMP1
Molecular Weight	54641 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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EGF-containing fibulin-like extracellular matrix protein 1, Extracellular protein S1-5, Fibrillin-like protein, Fibulin-3, FIBL-3, EFEMP1, FBLN3, FBNL

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Western blot analysis of EFEMP1 Antibody (N-term) in mouse lung tissue lysates (35 ug/lane). EFEMP1 (arrow) was detected using the purified Pab.

EFEMP1 Antibody (N-term) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with EFEMP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-EFEMP1 Antibody (N-term) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: HUVEC whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.