EGF R Recombinant Protein

SKU: orb1231148

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Description

Images & Validation

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Tested Applications	ELISA, WB
Application Notes	This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 94.7 kDa. The protein migrates as 110-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Source	HEK293 cells
Target	EGFR
Tag	Fc Tag
Molecular Weight	94.7 kDa
Protein Sequence	Leu 25 - Ser 645
Purity	>95% as determined by SDS-PAGE.

Storage & Handling

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Storage	Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	PBS, pH 7.4
Disclaimer	For research use only

Alternative Names

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EGFR,ERBB,ERBB1,HER1,PIG61,mENA

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Human EGF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Immobilized Human EGF, Tag Free at 10 ug/ml (100 uL/well) can bind Human EGF R, Fc Tag with a linear range of 0.078-2.5 ug/ml.

Immobilized Human EGF R, Fc Tag at 1 ug/ml (100 uL/well) can bind Cetuximab with a linear range of 0.1-2 ng/mL.

Quick Database Links

Gene Symbol

EGFR

UniProt

P00533

RefSeq (Protein):NP_005219

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_005219

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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