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EGFR Antibody
SKU: orb153505
Featured
Description
Images & Validation
−Item 1 of 7
| Tested Applications | FC, IHC, WB |
|---|---|
| Dilution range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 7A6F12 |
| Immunogen | Purified recombinant fragment of human EGFR (AA: 693-893) expressed in E. Coli. |
| Target | EGFR |
| Molecular Weight | 175kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Disclaimer | For research use only |
Alternative Names
−ERBB; HER1; mENA; ERBB1; PIG61
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Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of EGFR antibody
Flow cytometric analysis of A431 cells lysates using EGFR antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded endometrial cancer tissues using EGFR antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded esophageal cancer tissues using EGFR antibody
Western blot analysis of human EGFR using EGFR antibody
Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using EGFR antibody
Western blot analysis of A431 (Lane 1) Hela (Lane 2) cell lysate using EGFR antibody
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
EGFR Antibody (orb153505)
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