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EIF2A Antibody
Description
Images & Validation
−| Tested Applications | FC, IHC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Monkey, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 3A7B11 |
| Immunogen | Purified recombinant fragment of human EIF2A (AA: 448-576) expressed in E. Coli. |
| Target | EIF2A |
| Molecular Weight | 65kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of EIF2A antibody

Flow cytometric analysis of HepG2 cells lysates using EIF2A antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded cervical cancer tissues using EIF2A antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded bladder cancer tissues using EIF2A antibody

Western blot analysis of human EIF2A using EIF2A antibody

Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using EIF2A antibody

Western blot analysis of MCF-7 (Lane 1), PC-12 (Lane 2), HepG2 (Lane 3), Hela (Lane 4), Cos7 (Lane 5), K562 (Lane 6), Jurkat (Lane 7), A431 (Lane 8) NIH/3T3 (Lane 9) cell lysate using EIF2A antibody
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EIF2A Antibody (orb153544)
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