EIF3A Rabbit Polyclonal Antibody

SKU: orb6005

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Description

Research Area

Epigenetics and Nuclear Signaling

Images & Validation

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Item 1 of 2

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Porcine, Rabbit, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human eIF3A (801-900/1382aa)
Target	EIF3A
Molecular Weight	166 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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centrosomin homolog; cytoplasmic protein p167; eIF 3 theta; eIF-3-theta; EIF3; eIF3 p167; eIF3 p170; eIF3 p180; EIF3 p180 subunit; eIF3 p185; eIF3 theta; eIF3a; EIF3S10; eukaryotic translation initiation factor 3 subunit 10(theta 150 170kD); eukaryotic translation initiation factor 3 subunit 10(theta 170kD); eukaryotic translation initiation factor 3 subunit 10 170kDa; Eukaryotic translation initiation factor 3 subunit 10; eukaryotic translation initiation factor 3 subunit 10 theta 150 170kDa; Eukaryotic translation initiation factor 3 subunit A; P167; p180; p185; TIF32.

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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (eIF3A) Polyclonal Antibody, Unconjugated (orb6005) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Testis (Mouse) Lysate at 40 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-eIF3A (orb6005) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 166 kD, Observed band size: 166 kD.

Quick Database Links

Gene Symbol

EIF3A

UniProt

Q14152

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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