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EIF4A1 Antibody (N-term)
SKU: orb1929421
Description
Images & Validation
−Item 1 of 3
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000, IHC-P - 1:100-500 |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Monkey, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 46154 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
−DDX2A, EIF4A
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EIF4A1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-EIF4A1 Pab in K562 cell line lysates (35 ug/lane). EIF4A1 (arrow) was detected using the purified Pab.
Western blot analysis of EIF4A1 (arrow) using rabbit polyclonal EIF4A1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4A1 gene (Lane 2).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
EIF4A1 Antibody (N-term) (orb1929421)
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